Journal: Journal of Immunology Research

Article Title: Human Immune System Increases Breast Cancer-Induced Osteoblastic Bone Growth in a Humanized Mouse Model without Affecting Normal Bone

doi: 10.1155/2019/4260987

Figure Lengend Snippet: Tumor growth and TILs in NOG and huNOG mice. (a) Representative HE-stained sections of the tumor-bearing tibias of NOG and huNOG mice, magnification 5x. (b) Quantitation of the tumor area (only tumor cells) in the bone marrow (mm 2 , mean ± IQR25%±min/max). Statistical analysis was performed using ANOVA and pairwise comparisons using Dunnett's test. Statistical significance is marked as ∗ p < 0.05. (c) Representative images of CD3 (T cells), CD4 (T helper cells), CD8 (cytotoxic T cells), and CD20 (B cells) in tumors growing in the bone, magnification 20x. (d) Representative images of immune checkpoint inhibitors PD-L1 (programmed death-ligand 1), PD-1 (programmed cell death protein 1), and CTLA-4 (cytotoxic T lymphocyte-associated protein 4) in the spleen, lymph nodes, and tumors, magnification 20x.

Article Snippet: The following primary antibodies were used: CD45 (common leukocyte marker, 2B11+PD7/26/16), CD3 (T cell, BSR10), CD4 (T helper cell, BSR4), CD8 (cytotoxic T cell, BSR5), CD20 (B cell, BSR6), PD-1 (programmed cell death protein 1, BSR1), PD-L1 (programmed death-ligand 1, ZR3, all from Nordic BioSite), and CTLA-4 (cytotoxic T lymphocyte-associated protein 4, BSB-88, BioSB).

Techniques: Staining, Quantitation Assay

Journal: Immunotherapy Advances

Article Title: Impact of Fc receptors and host characteristics on myeloid phagocytic response to rituximab-treated 3D-cultured B-cell lymphoma

doi: 10.1093/immadv/ltad025

Figure Lengend Snippet: Phagocytosis of RTX-opsonized 3D-cultured B-cell lymphoma. The percentage of primary monocytes from healthy donors (A), and female and male healthy donors (B) phagocytosing B-cell lymphoma (Raji) spheroids opsonized with different RTX-isotypes, isotype control antibodies, or without antibody treatment (w/o) analyzed by flow cytometry. Data are shown as mean ± SEM ( n = 30–35 donors). Asterisks represent statistically significant difference between RTX and isotype control-treated spheroids. * P < 0.05, **** P < 0.0001.

Article Snippet: CD20+ B-cell lymphoma cells, Raji, originating from human Burkitt’s B-cell lymphoma, authenticated by STR-profiling (Microsynth AG), were cultured in complete RPMI at 37°C in a humidified chamber under 5% CO 2 .

Techniques: Cell Culture, Control, Flow Cytometry

Journal: Immunotherapy Advances

Article Title: Impact of Fc receptors and host characteristics on myeloid phagocytic response to rituximab-treated 3D-cultured B-cell lymphoma

doi: 10.1093/immadv/ltad025

Figure Lengend Snippet: Fluorescence confocal images of RTX-opsonized B-cell lymphoma spheroids co-cultured with primary monocytes. RTX-IgG1, RTX-IgG3, or RTX-IgA1 opsonized B-cell lymphoma spheroids (Raji) stained with CFSE (green) and primary monocytes from healthy donor stained with anti-CD14 PE (magenta). Results are from one representative experiment out of three. Scale bar = 150 μm, magnification 20×.

Article Snippet: CD20+ B-cell lymphoma cells, Raji, originating from human Burkitt’s B-cell lymphoma, authenticated by STR-profiling (Microsynth AG), were cultured in complete RPMI at 37°C in a humidified chamber under 5% CO 2 .

Techniques: Fluorescence, Cell Culture, Staining

Journal: Immunotherapy Advances

Article Title: Impact of Fc receptors and host characteristics on myeloid phagocytic response to rituximab-treated 3D-cultured B-cell lymphoma

doi: 10.1093/immadv/ltad025

Figure Lengend Snippet: Blocking of FcγRs and phagocytosis of RTX-treated 3D-cultured B-cell lymphoma. Primary monocytes from healthy donor were left untreated (plain bars), or treated with mAb to FcγRI (clone 10.1), FcγRII (clone AT10) or FcγRIII (clone 3G8) (dotted bars) prior co-culture with RTX-IgG1 (white bars) or RTX-IgG3 (red bars) opsonized B-cell lymphoma (Raji) spheroids. Data are presented as the mean percentage phagocytosis ± SEM of one representative out of three independent experiments. Asterisks represent statistically significant difference between isotype control and RTX-treated spheroids, or FcγR-blocked and untreated monocytes phagocytosing RTX-treated treated spheroids. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: CD20+ B-cell lymphoma cells, Raji, originating from human Burkitt’s B-cell lymphoma, authenticated by STR-profiling (Microsynth AG), were cultured in complete RPMI at 37°C in a humidified chamber under 5% CO 2 .

Techniques: Blocking Assay, Cell Culture, Co-Culture Assay, Control